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    NC文章详解 | 鼠成纤维细胞单细胞分析发现成纤维细胞在心肌细胞成熟中起关键作用

    Cardiac maturation lays the foundation for postnatal heart development and disease, yet little is known about the contributions of the microenvironment to cardiomyocyte maturation. By integrating single-cell RNA-sequencing data of mouse hearts at multiple postnatal stages, we construct cellular interactomes and regulatory signaling networks. Here we report switching of fibroblast subtypes from a neonatal to adult state and this drives cardiomyocyte maturation. Molecular and functional maturation of neonatal mouse cardiomyocytes and human embryonic stem cell-derived cardiomyocytes are considerably enhanced upon co-culture with corresponding adult cardiac fibroblasts. Further, single-cell analysis of in vivo and in vitro cardiomyocyte maturation trajectories identify highly conserved signaling pathways, pharmacological targeting of which substantially delays cardiomyocyte maturation in postnatal hearts, and markedly enhances cardiomyocyte proliferation and improves cardiac function in infarcted hearts. Together, we identify cardiac fibroblasts as a key constituent in the microenvironment promoting cardiomyocyte maturation, providing insights into how the manipulation of cardiomyocyte maturity may impact on disease development and regeneration.

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    红队第5篇:MS12-020蓝屏漏洞在实战中的巧用

    大家好,上期分享了一篇Shiro Oracle Padding反序列化漏洞无key的实战文章,这期讲一个MS12-020蓝屏漏洞的真实利用过程。这个案例源于2013年我在读研期间,印象是比较深的。在学校期间,我偶尔会帮网络部那边处理一些网站故障,还帮忙修补过安全漏洞。在那个年代,大学网站的漏洞是非常多的,基本上没有什么WAF设备防护。期间有一个大学网站大概是长时间没有人用,崩溃了,访问一直是卡死状态,服务器密码学校那边也不记得了。于是我就来了一顿操作,帮忙恢复了一下,中间也踩了不少坑。首先肯定是要想办法拿到服务器权限,然后帮学校把密码读出来,下面凭着记忆,把过程写出来。

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