SingleCellExperiment
是单细胞转录组数据的基础包,大部分其它单细胞转录组数据处理的包都依赖于它,就跟芯片数据里面的ExpressionSet
对象一样,需要拼了命的理解透,才有可能做好数据分析。
最近MAC升级就死机,好不容易通过谷歌救活了,但是R里面安装SingleCellExperiment
包却开始报错,
## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("SingleCellExperiment")
Bioconductor version 3.5 (BiocInstaller 1.26.1), ?biocLite for help
A newer version of Bioconductor is available for this version of R, ?BiocUpgrade
for help
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.5 (BiocInstaller 1.26.1), R 3.4.3 (2017-11-30).
Installing package(s) 'SingleCellExperiment'
Warning message:
package 'SingleCellExperiment' is not available (for R version 3.4.3)
按照我当初总结好的R包安装大全,R包终极解决方案 (请务必点击仔细阅读!!!)
下载它的macos版本压缩文件,直接解压到R的库目录,最后成功的安装了这个包
suppressPackageStartupMessages(library(scater))
# Error: This is R 3.4.3, package 'SingleCellExperiment' needs >= 3.5
很诡异的,当时硬是想不通为啥子,就去上海聚会了,放松了五天,回来检查了我的浏览器记录,发现这个SingleCellExperiment
居然是有两个url,如下:
https://bioconductor.org/packages/release/bioc/html/SingleCellExperiment.html
https://bioconductor.org/packages/3.7/bioc/html/SingleCellExperiment.html
我有点诧异,一般来说,用最新版的即可。我进入仔细对比,发现依赖于3.7版本的bioconductor的那个包介绍页面里面写着依赖于 R (>= 3.5)
顿时尴尬了,现在是 2018年01月15日21:51:39 ,最新版的R还是3.4呀~!!
原来是当初我下载的SingleCellExperiment
版本太超前了,尽管我把它挪到了library的目录,我的R也无法使用它,我删除了该目录,重新下载了一个!
wget https://bioconductor.org/packages/release/bioc/bin/macosx/el-capitan/contrib/3.4/SingleCellExperiment_1.0.0.tgz
这个时候这个包可以被载入,但是仍然报错
本以为安装成功了,也可以载入,就万事大吉了,事实证明我还是太年轻
suppressPackageStartupMessages(library(scater))
data("sc_example_counts")
data("sc_example_cell_info")
example_sce <- SingleCellExperiment(
assays = list(counts = sc_example_counts), colData = sc_example_cell_info)
#Error in checkSlotAssignment(object, name, value) :
# assignment of an object of class "GRangesList" is not valid for slot 'rowRanges' in an object of class "SingleCellExperiment"; is(value, "GenomicRanges_OR_GRangesList") is not TRUE
很明显仍然是包不兼容的问题。
我又回过头想了想,为什么不能自然而然的安装包呢?看到了升级选项:
biocLite("BiocUpgrade")
> source("https://bioconductor.org/biocLite.R")
Bioconductor version 3.6 (BiocInstaller 1.28.0), ?biocLite for help
> biocLite("SingleCellExperiment")
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.6 (BiocInstaller 1.28.0), R 3.4.3 (2017-11-30).
Installing package(s) 'SingleCellExperiment'
trying URL 'https://bioconductor.org/packages/3.6/bioc/bin/macosx/el-capitan/contrib/3.4/SingleCellExperiment_1.0.0.tgz'
Content type 'application/x-gzip' length 857294 bytes (837 KB)
==================================================
downloaded 837 KB
学习之路,任重而道远!
切勿纠结,放松两天,也许思路就慢慢理顺了!